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siPOOLs用于药物靶点筛选研究(RNAi)
对于靶向基因沉默,RNA干扰(RNAi)具有易于操作、快速结果、高效率、广泛适用于各种细胞类型的多重优 势。其具有瞬转效应和剂量依赖效应,这点与小分子非常相似。然而,目前的siRNA试剂其特异性和基因沉默 效率可变,阻碍了它们作为药物发现和基因研究工具的应用。
siTOOLs Biotech由Michael Hannus博士和Gunter Meister教授于2013年9月成立,由德国雷根斯堡大学 和Intana Bioscience制药服务公司联合运营。目前推出有3个产品系列:(1)siPOOLs:一种siRNA混合物,有 效增加基因沉默特异性,“稀释”传统RNA干扰试剂的脱靶效应。(2)raPOOLs:一种稳健的RNA亲和纯化方 案,适用于基因功能和相互作用的生物化学研究。(3)riboPOOLs:适合任意物种的核糖体RNA去除试剂,高效且经济。
可靠表型的特异性基因沉默工具
siPOOLs是一款经过优化设计的高复杂性的包含30条siRNA的混合物,经证明可有效消除脱靶效应,并提高结果的可靠性(Hannus et al., 2014)。 Pack Hunter (pooling) 方法通过将当个siRNA的 浓度稀释到刺激表型的阈值以下来对抗单个siRNA的脱靶。 借助专有的设计算法,siPOOLs中的siRNA序列经 过优化,以实现最大的转录本覆盖率,高效杂交并 对旁系同源基因进行过滤,从而实现高效和特异性 的基因沉默。
siPOOLs产品优势
使用简单快捷:siPOOLs与多种转染试剂兼容,几天内就能看 到结果。
高度特异性且有效:siPOOLs在标准细胞系中将脱靶率降低5-25 倍,且在1-3 nM下实现基因敲低率≥70%。
一致的表型:与siRNA相反,由序列非依赖性的siPOOL产生 的表型高度一致。
确保经过检验:通过RT-qPCR进行siPOOL验证,如果在 转染下敲低率低于70%,则有可能重新设计。
使用注释进行定制设计:专业的设计,确保优化热力学特性且避免旁系 同源基因。
HPLC纯化且无毒:所有siPOOL均经过HPLC纯化,可降低污染物 和副作用的风险。
关键问题:siRNAs的脱靶效应
科学家们一直在使用RNA干扰(RNAi)作为研究基因功能的快速有效的工具。然而,短干扰RNA(siRNA)的脱靶效应和可变性能仍然是一个令人头疼的缺点,在验证工作中消耗了宝贵的时间和资源。
siRNA通常与靶RNA转录本互补结合,通过RNAi机制指导其降解。脱靶效应主要是由siRNA模拟内源性 基因调节因子microRNA(miRNA)引起的。由于miRNA只需要6个碱基种子匹配到3'非翻译区(UTR)即可触 发转录本下调,因此siRNA在通过这种机制处理时可以改变许多意外靶标的表达。
siPOOLs如何提高特异性
单个siRNA或含有3-4个siRNA的低复杂度siRNA库,经常击中多个脱靶基因并表现出易变的靶基因敲低。 siPOOL是高度复杂且确定的30个siRNA池,每个siRNA以皮摩尔工作浓度存在。因此, · 稀释了每个siRNA的脱靶特征,提高了靶向特异性。 · 确保了靶基因的协同敲低,产生更稳健、更可靠的结果。
使用siPOOL具有更高的特异性
特异性好的试剂应仅影响其靶标基因。
HeLa细胞中微阵列的表达谱分析显示,单个siRNA可以诱导许多脱靶基因(红点),而针对同一靶基因(绿 点)并包含非特异性siRNA的siPOOL大大降低了脱靶效应。
通过siPOOL实现更好的重现性和有效的敲低效率
普通siRNA的敲低效率差异很大。
与单个siRNA相比,针对同一基因的siPOOLs具有相似的敲低效率,表明具有更高的稳健性和可重复性(左 图和中图:靶RNA水平的实时定量PCR测量的相关图)。 siPOOLs也表现出有效的基因敲低。在常用的细胞系中,许多基因通常在1 nM浓度下实现75-98%的基 因敲低效率(右图)。
您可以信赖的表型
如果RNAi效果可靠且特异,靶向同一基因的两种试剂应产生相似的表型。
每个基因分别使用两个siPOOL池,一个来自我们的人类激酶siPOOL文库,一个是市售的每个基因含3条 siRNAs的文库,科学家在A549细胞中筛选了36个基因并检测了细胞活力。仅siPOOLs产生了较好的一致性表型。
siTOOLs生物技术始终致力于帮助学术研究人员、科学家、制药/生物技术公司和RNAi筛选人员。目前,已帮助许多论文 发表在《自然》、《细胞》、《自然医学》、《科学报告》等顶级期刊上。期待与您的合作,我们将为您的RNA研究提供量身定制 的分子工具。
参考文献
Hannus M. et al. (2014) siPools: highly complex but accurately defined siRNA pools eliminate off-target effects. Nucleic Acids Res 42(12): 8049-61
Zimmermann et al. (2023) Cold Atmospheric Plasma Triggers Apoptosis via the Unfolded Protein Response in Melanoma Cells MDPI
Müller et al. (2023) Plakophilin 3 facilitates G1/S phase transition and enhances proliferation by capturing RB protein in the cytoplasm and promoting EGFR signaling Cell Reports
Luo et al. (2023) The long non-coding RNA LINC00958 is induced in psoriasis epidermis and modulates epidermal proliferation Journal of Investigative Dermatology
Staebler et al. (2023) MIA/CD-RAP Regulates MMP13 and Is a Potential New Disease-Modifying Target for Osteoarthritis Therapy MDPI
Hertel et al. (2022) USP32-regulated LAMTOR1 ubiquitination impacts mTORC1 activation and autophagy induction Cell Reports
Yavon et al. (2022) High-throughput morphometric and transcriptomic profiling uncovers composition of naïve and sensory-deprived cortical cholinergic VIP/CHAT neurons The EMBO Journal
Kim et al. (2022) Screening of the siGPCR library in combination with cisplatin against lung cancers Scientific Reports
Mitani et al. (2022) SNAP23-Mediated Perturbation of Cholesterol-Enriched Membrane Microdomain Promotes Extracellular Vesicle Production in Src-Activated Cancer Cells Biological and Pharmaceutical Bulletin
Blümel et al. (2022) Primary cilia contribute to the aggressiveness of atypical teratoid/rhabdoid tumors Cell Death & Disease
Rovira et al. (2022) The lysosomal proteome of senescent cells contributes to the senescence secretome Aging Cell
Koch et al. (2022) Targeting the Retinoblastoma/E2F repressive complex by CDK4/6 inhibitors amplifies oncolytic potency of an oncolytic adenovirus Nature Communications
Schaefer et al. (2022) Global and precise identification of functionalmiRNA targets in mESCs by integrative analysis EMBO Reports
Kerstin Dörner et al. (2022) Genome-wide RNAi screen identifies novel players in human 60S subunit biogenesis including key enzymes of polyamine metabolism Nucleic Acids Research
Bartl et al. (2022) The HHIP-AS1 lncRNA promotes tumorigenicity through stabilization of dynein complex 1 in human SHH-driven tumors Nature Communications
Banas et al. (2022) The Interplay of NEAT1 and miR-339-5p Influences on Mesangial Gene Expression and Function in Various Diabetic-Associated Injury Models non-coding RNA
Mestre-Farràs et al. (2022) Melanoma RBPome identification reveals PDIA6 as an unconventional RNA-binding protein involved in metastasis Nucleic Acids Research
Dessauges et al. (2022) Optogenetic actuator – ERK biosensor circuits identify MAPK network nodes that shape ERK dynamics Molecular Systems Biology
Falke et al. (2022) Knockdown of the stem cell marker Musashi-1 inhibits endometrial cancer growth and sensitizes cells to radiation Stem Cell Research & Therapy
Gutierrez-Prat et al. (2022) DUSP4 protects BRAF- and NRAS-mutant melanoma from oncogene overdose through modulation of MITF Life Science Alliance
Lechner et al. (2022) Target deconvolution of HDAC pharmacopoeia reveals MBLAC2 as common off-target Nature Chemical Biology
Daniel Kummer et al. (2022) A JAM-A–tetraspanin–αvβ5 integrin complex regulates contact inhibition of locomotion Journal of Cell Biology
Delicato, A. et al (2022) YB-1 Oncoprotein Controls PI3K/Akt Pathway by Reducing Pten Protein Level Genes
Szachnowski et al. (2022) Transcriptomic landscapes of SARS-CoV-2-infected and bystander lung cells reveal a selective upregulation of NF-κB-dependent coding and non-coding proviral transcripts bioRxiv - preprint
Hauffe, L. et al. (2022) Eukaryotic translation initiation factor 4E binding protein 1 (EIF4EBP1) expression in glioblastoma is driven by ETS1- and MYBL2-dependent transcriptional activation Cell Death Discovery
Till Braun et al. (2022) Non-canonical function of AGO2 augments T-cell receptor signaling in T-cell prolymphocytic leukemia Amercian Association for Cancer Research
Karabid et al. (2022) Angpt2/Tie2 autostimulatory loop controls tumorigenesis EMBO Mol Med
Kerstin Dörner et al. (2022) Genome-wide RNAi screen identifies novel players in human 60S subunit biogenesis including key enzymes of polyamine metabolism Nucleic Acids Research
X. Li et al. (2022) 5’isomiR-183-5p|+2 Elicits Tumor Suppressor Activity in a Negative Feedback Loop with E2F1 Journal of Experimental & Clinical Cancer Research - preprint -
Thölmann et al. (2022) JAM-A interacts with α3β1 integrin and tetraspanins CD151 and CD9 to regulate collective cell migration of polarized epithelial cells Cellular and Molecular Life Sciences
Thea Reinkens et al. (2021) Ago-RIP Sequencing Identifies New MicroRNA-449a-5p Target Genes Increasing Sorafenib Efficacy in Hepatocellular Carcinoma Journal Of Cancer
Weihua Qin et. Al (2022) Probing protein ubiquitination in live cells bioRxiv - preprint
Belitškin et al. (2021) Hepsin regulates TGFβ signaling via fibronectin proteolysis EMBO reports
Q. Zou et al (2021) Utility of single-shot compressed sensing cardiac magnetic resonance cine imaging for assessment of biventricular function in free-breathing and arrhythmic pediatric patients International Journal of Cardiology
J. Laisney et al (2021) Delivery of short hairpin RNA in the neotropical brown stink bug, Euschistus heros, using a composite nanomaterial Pesticide Biochemistry and Physiology
Indacochea et al (2021) Cold-inducible RNA bindi ng protein promotes breast cancer cell maligna ncy by regulating Cys tatin C levels Cold Spring Harbor Laboratory Press
Khan et al (2021) Developing Tumor Radiosensitivity Signatures Using LncRNAs Radiation Research
Ghodke et al (2021) AHNAK controls 53BP1-mediated p53 response by restraining 53BP1 oligomerization and phase separation Molecular Cell
Gaza et al (2021) Identification of novel targets of miR-622 in hepatocellular carcinoma reveals common regulation of cooperating genes and outlines the oncogenic role of zinc finger CCHC-type containing 11 Neoplasia
Indacochea et al (2021) Cold-inducible RNA binding protein promotes breast cancer cell maligna ncy by regulating Cys tatin C levels Cold Spring Harbor Laboratory Pres
Mercier et al (2020) Endosomal membrane tension regulates ESCRT-III-dependent intra-lumenal vesicle formation Nature Cell Biology
Ghosh et al (2020) Prevention of dsRNA-induced interferon signaling by AGO1x is linked to breast cancer cell proliferation The EMBO Journal
Dhamija et. al (2020) A pan-cancer analysis reveals nonstop extension mutations causing SMAD4 tumour suppressor degradation Nature Cell Biology
Gandhi et. al (2020) The lncRNA lincNMR regulates nucleotide metabolism via a YBX1 - RRM2 axis in cancer Nature Communications
Stephan et. al (2020) MICOS assembly controls mitochondrial inner membrane remodeling and crista junction redistribution to mediate cristae formation The EMBO journal
Liebig et. al (2020) HuRdling Senescence: HuR Breaks BRAF-Induced Senescence in Melanocytes and Supports Melanoma Growth. MDPI Cancers
Prasad et. al (2020) The UPR sensor IRE1α and the adenovirus E3-19K glycoprotein sustain persistent and lytic infections. Nature Communications
Marchetto et. al (2020) Oncogenic hijacking of a developmental transcription factor evokes vulnerability toward oxidative stress in Ewing sarcoma. Nature Communications
Larios, J. et. al (2020) ALIX- and ESCRT-III–dependent sorting of tetraspanins to exosomes. JCB
Mahli, A. et. al (2019) Bone Morphogenic Protein-8B Expression is Induced in Steatotic Hepatocytes and Promotes Hepatic Steatosis and Inflammation in Vitro. Cells
Renner, K. et. al (2019) Restricting Glycolysis Preserves T Cell Effector Functions and Augments Checkpoint Therapy. Cell Rep
Werner, S. et. al (2019) MRTF-A controls myofibroblastic differentiation of human multipotent stromal cells and their tumour-supporting function in xenograft models. Scientific Reports
Braun, C. et. al (2019) Inhibition of peptidyl-prolyl isomerase (PIN1) and BRAF signaling to target melanoma. Am J Transl Res
Kappelmann-Fenzl, M. et. al (2019) C-Jun drives melanoma progression in PTEN wild type melanoma cells. Cell Death Dis
Simchovitz, A et. al (2019) NEAT1 is overexpressed in Parkinson’s disease substantia nigra and confers drug-inducible neuroprotection from oxidative stress. FASEB J
Mahli, A et. al (2019) Bone Morphogenetic Protein-8B Expression is Induced in Steatotic Hepatocytes and Promotes Hepatic Steatosis and Inflammation In Vitro. Cells
Dietrich, P et. al (2019) The Delta Subunit of Rod-Specific Photoreceptor cGMP Phosphodiesterase (PDE6D) Contributes to Hepatocellular Carcinoma Progression. Cancers
Feuerer, L et. al (2019) Role of MIA (melanoma inhibitory activity) in melanocyte senescence. Pigment Cell Melanoma Res
Ziegler, C. et. al (2019) The long non‐coding RNA LINC00941 and SPRR5 are novel regulators of human epidermal homeostasis. EMBO Rep
Choi, K. et. al (2019) Binary Targeting of siRNA to Hematologic Cancer Cells In Vivo Using Layer‐by‐Layer Nanoparticles. Adv Funct Mater
Spies, J. et. al (2019) 53BP1 nuclear bodies enforce replication timing at under-replicated DNA to limit heritable DNA damage. Nat Cell Biol
Dietrich, P. et al. (2019) Neuroblastoma RAS Viral Oncogene Homolog (NRAS) Is a Novel Prognostic Marker and Contributes to Sorafenib Resistance in Hepatocellular Carcinoma. Neoplasia
Vendramin, R. et al. (2018) SAMMSON fosters cancer cell fitness by concertedly enhancing mitochondrial and cytosolic translation. Nat Struct Mol Biol
Coscia, F. et al. (2018) Multi-level Proteomics Identifies CT45 as a Chemosensitivity Mediator and Immunotherapy Target in Ovarian Cancer. Cell
Kästle, M. et al. FKBP51 modulates steroid sensitivity and NFκB signalling: A novel anti-inflammatory drug target. Eur J Immunol
Stieglitz, D. et al. BMP6-induced modulation of the tumor micro-milieu. Oncogene 10.1038/s41388-018-0475-x (2018)
Weber, A., Schwarz, S. C. et al. Epigenome-wide DNA methylation profiling in Progressive Supranuclear Palsy reveals major changes at DLX1. Nat Commun 9, 2929 (2018)
Noethel, B. et al. Transition of responsive mechanosensitive elements from focal adhesions to adherens junctions upon epithelial differentiation. Mol Biol Cell 10.1091/mbc.E17-06-0387(2018)
Prestigiacomo, V., and Suter-Dick, L. Nrf2 protects stellate cells from Smad-dependent cell activation. PLoS One 13, e0201044 (2018)
Vallin, B. et al. Novel short isoforms of adenylyl cyclase as negative regulators of cAMP production. Biochim Biophys Acta - Mol Cell Res 1865: 1326–1340 (2018)
Rietscher, K. et al.14-3-3 Proteins Regulate Desmosomal Adhesion via Plakophilins.” Journal of cell science 131(10). (2018).
Jiang, P. et al. Genome-Scale Signatures of Gene Interaction from Compound Screens Predict Clinical Efficacy of Targeted Cancer Therapies.” Cell Systems 6(3): 343–354.e5. (2018).
So, D. et al. Cervical Cancer Is Addicted to SIRT1 Disarming the AIM2 Antiviral Defense. Oncogene (2018).
Klingenberg, M. et al. The lncRNA CASC9 and RNA Binding Protein HNRNPL Form a Complex and Co-Regulate Genes Linked to AKT Signaling. Hepatology (2018).
Polycarpou-Schwarz, M. et al. The Cancer-Associated Microprotein CASIMO1 Controls Cell Proliferation and Interacts with Squalene Epoxidase Modulating Lipid Droplet Formation. Oncogene (2018)
Zeiner, P. S. et al. CD74 regulates complexity of tumor cell HLA class II peptidome in brain metastasis and is a positive prognostic marker for patient survival. Acta Neuropathol. Commun. 6, 18 (2018).
Ziegelmann, B et al. Lithium chloride effectively kills the honey bee parasite Varroa destructor by a systemic mode of action. Scientific Reports 8(1), 683 (2018)
Dietrich, P. et al. Wild type Kirsten rat sarcoma is a novel microRNA-622-regulated therapeutic target for hepatocellular carcinoma and contributes to sorafenib resistance. Gut (2017)
Dietrich, P. et al. Wild-type KRAS is a novel therapeutic target for melanoma contributing to primary and acquired resistance to BRAF inhibition. Oncogene (2017)
Kaller, M. et al. Loss of p53-inducible long non-coding RNA LINC01021 increases chemosensitivity. Oncotarget 8, 102783–102800 (2017).
Ilina, E. I. et al. Effects of soluble CPE on glioma cell migration are associated with mTOR activation and enhanced glucose flux. Oncotarget 8, 67567–67591 (2017).
Goyal, A. et al. A cautionary tale of sense-antisense gene pairs: independent regulation despite inverse correlation of expression. Nucleic Acids Research (2017)
Steinbach, A. et al. ERAP1 overexpression in HPV-induced malignancies: A possible novel immune evasion mechanism. Oncoimmunology 6, e1336594 (2017).
Sluch, V. M. et al. Enhanced Stem Cell Differentiation and Immunopurification of Genome Engineered Human Retinal Ganglion Cells. STEM CELLS Translational Medicine. (2017) doi:10.1002/sctm.17-0059
Armento, A. et al. Carboxypeptidase E transmits its anti-migratory function in glioma cells via transcriptional regulation of cell architecture and motility regulating factors. International Journal of Oncology 51(2), 702-714 (2017)
Nötzold, L. et al. The long non-coding RNA LINC00152 is essential for cell cycle progression through mitosis in HeLa cells. Scientific Reports 7, 2265 (2017) Also cites raPOOL
Welsbie, D. S. et al. Enhanced Functional Genomic Screening Identifies Novel Mediators of Dual Leucine Zipper Kinase-Dependent Injury Signaling in Neurons. Neuron 94(6), 1142–1154.e6 (2017)
Ruivo, M. T. G. et al. Host AMPK Is a Modulator of Plasmodium Liver Infection. Cell Rep. 1–7 (2017).
Treiber, T. et al. A Compendium of RNA-Binding Proteins that Regulate MicroRNA Biogenesis. Mol. Cell 66, 270–284.e13 (2017).
Seiler, J. et al. The lncRNA VELUCT strongly regulates viability of lung cancer cells despite its extremely low abundance. Nucleic Acids Res. (2017)
Goyal, A. et al. Challenges of CRISPR/Cas9 applications for long non-coding RNA genes. Nucleic Acids Res. (2016)
Ott, C. A. et al. Induction of exportin-5 expression during melanoma development supports the cellular behavior of human malignant melanoma cells. Oncotarget 7, 62292–62304 (2016).
Adriaens, C. et al. p53 induces formation of NEAT1 lncRNA-containing paraspeckles that modulate replication stress response and chemosensitivity. Nat. Med. (2016).
Coll-Bonfill, N. et al. Slug is increased in vascular remodeling and induces a smooth muscle cell proliferative phenotype. PLoS One 11, 1–21 (2016).
Kordaß, T. et al. SOX5 is involved in balanced MITF regulation in human melanoma cells. BMC Med. Genomics 9, 10 (2016).
Hauptmann, J. et al. Biochemical isolation of Argonaute protein complexes by Ago-APP. Proc. Natl. Acad. Sci. 112, 11841–11845 (2015).
Blirando, K. et al. The stellate vascular smooth muscle cell phenotype is induced by IL-1β via the secretion of PGE2 and subsequent cAMP-dependent protein kinase A activation. Biochim. Biophys. Acta. 1853, 3235–3247 (2015).
Tuncay, H. et al. JAM-A regulates cortical dynein localization through Cdc42 to control planar spindle orientation during mitosis. Nat. Commun. 6, 8128 (2015).
Schraivogel, D. et al. Importin-β facilitates nuclear import of human GW proteins and balances cytoplasmic gene silencing protein levels. Nucleic Acids Res. (2015)
Schönemann L. et al.: Reconstitution of CPSF active in polyadenylation: recognition of the polyadenylation signal by WDR33. Genes Dev. 28(21), 2381-93 (2014)
Prasad, V. et al. Chemical induction of unfolded protein response enhances cancer cell killing through lytic virus infection. J. Virol. 88, 13086–13098 (2014).
Patrick C.H. Lo, PhD: siRNAs: Jumping in the Pool to Avoid Hitting Innocent Bystanders. BioTechniques (2014)
Thalyana S. Advances in RNAi Tools and Technologies. Genetic Engineering & Biotechnology News. April 33(8): 20-22, 24 (2013)
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