NEXTFLEX®快速DNA测序试剂盒2.0设计用于使用1 ng–1µg片段DNA构建约3小时的DNA文库。不建议将此试剂盒用于FFPE样品。该试剂盒可用于使用Illumina®平台制备用于单端或成对端测序的多重文库。

GENERAL INFORMATION

Product Overview

The NEXTFLEX® Rapid DNA-Seq Kit 2.0 is designed for an approximately 3-hour DNA library construction with 1 ng – 1 µg of fragmented DNA. This kit is not recommended for FFPE samples. The kit can be used to prepare multiplexed libraries for single or paired end sequencing using Illumina® platforms. In addition, the availability of up to 1536 unique adapter barcodes facilitates high-throughput applications. There are five main steps involved in preparing DNA for sequencing: DNA extraction, DNA fragmentation, DNA end repair / adenylation, adapter ligation, and PCR amplification. The NEXTFLEX® Rapid 2.0 DNA Seq Kit contains the necessary material to take the user’s purified and fragmented DNA through preparation and amplification for loading onto flow cells for sequencing.